Cyclocybe aegerita Polysaccharide Powder

Hot-water extraction of the Cyclocybe aegerita fruiting body gives this polysaccharide powder, the water-soluble glucan and heteropolysaccharide fraction of the black poplar mushroom, separated from the nutty aroma and the chitin-rich wall. It is purified, dried and milled to a fine light yellow powder.

Polysaccharide content is ≥30% by UV-Vis (phenol–sulfuric, as glucose), moisture and total ash are each ≤5.0%, and the powder is milled to 80 mesh. It dissolves in water and is supplied for dietary supplements, functional beverages and functional foods, with the method stated beside the figure.

SKU: BOT-BPS-0043 Category: Botanical Polysaccharides Tags: Dry Blends & Premixes, Mushroom & Fungi, Polysaccharides, Water Extracted, Water Soluble, Water-Extracted Fractions

Cyclocybe aegerita Polysaccharide Identity

Cyclocybe aegerita polysaccharide powder is made from the fruiting body of Cyclocybe aegerita (V.Brig.) Vizzini, the black poplar mushroom, also sold as velvet pioppino or yanagi-matsutake. The fruiting body is the part used. The same fungus appears in older literature as Agrocybe aegerita and Agrocybe cylindracea, so a search under those names returns work on the same organism.

In nature the fungus decays poplar and other hardwood, and in cultivation it is grown on hardwood sawdust blocks. The Cyclocybe aegerita fruiting body has a brown cap and a stout stem with a ring, and it is valued for a firm texture and a nutty aroma. That aroma comes from volatile compounds that dissolve poorly in water and are largely lost during extraction and drying.

The polysaccharides reported for Cyclocybe aegerita are β-glucans, with β-(1→3) and β-(1→6) linkages, and heteropolysaccharides that contain mannose, galactose and fucose in smaller shares. The mushroom also stores glycogen, an α-glucan, and builds its cell wall with chitin. Hot-water extraction of Cyclocybe aegerita takes out the soluble and swellable polymers, and ethanol precipitation concentrates them.

Cultivated fruiting bodies are harvested at the stage when the cap is still closed, then dried and sorted. Cap opening and drying history change the dry weight and sugar balance of the starting material, so extraction starts from dried, sorted fruiting body.

No other Cyclocybe aegerita product is currently listed in the catalog, so this powder is the only form of the mushroom that Essencefield offers.

Polysaccharide Content & Test Method

For Cyclocybe aegerita the content is stated as polysaccharides ≥30% by UV-Vis, a reading of total carbohydrate. Hot sulfuric acid hydrolyses every sugar-bearing polymer in the sample, phenol converts the released sugars into a colored product, and absorbance is compared with glucose, so the value is a glucose equivalent.

In Cyclocybe aegerita, glycogen and β-glucan release the same glucose, the assay cannot separate them. A black poplar mushroom extract could reach a UV-Vis percentage of 30% while holding a lower β-glucan share, and a figure quoted as β-glucan must come from an enzymatic method that subtracts α-glucan from total glucan. No such value is claimed here.

Hetero-polysaccharides that carry galactose, mannose or fucose respond to the reaction a little differently from glucose, which adds spread to the result. For that reason the standard curve is prepared fresh and run under the same acid and heating conditions as the sample.

Because the mushroom is grown on sawdust, the substrate is the main risk to the number. Cellulose or starch carried over from sawdust and supplements can add sugar to the reading, so the fruiting body is separated cleanly from the substrate at harvest and the purified powder, not the starting biomass, is the sample that carries the percentage.

Gravimetric ethanol-precipitate weights would include protein, and enzymatic assays are not used for this grade. Compare the number only with other UV-Vis values.

Typical Specifications

Specification Item	Product Information
Latin Name	Cyclocybe aegerita
Plant Part	Mushroom & Fungi
Processing	Water Extraction & Purification
Common Name	Black poplar mushroom (Yanagi-matsutake)
Ingredient Type	Polysaccharide Extract
Polysaccharide Type	β-glucans and heteropolysaccharides (fungal)
Content	Polysaccharides ≥30% (UV-Vis, as glucose)
Appearance	Light Yellow powder
Solubility / Dispersibility	Water Soluble
Quality Parameters
Moisture	≤5.0% (LOD)
Total Ash	≤5.0%
Particle Size	80 mesh
Heavy Metals	Lead ≤2 ppm · Arsenic ≤1 ppm · Cadmium ≤1 ppm · Mercury ≤0.1 ppm
Microbiology	TPC ≤1,000 cfu/g · Yeast & Mold ≤100 cfu/g · E. coli Negative · Salmonella Negative/25 g
Shelf Life	24 months in unopened original packaging, cool and dry storage

Cyclocybe aegerita Polysaccharide Solution Behavior

Warm water turns Cyclocybe aegerita polysaccharide into a pale yellow solution that carries a faint haze. The swollen, branched glucans give modest viscosity that grows with concentration. Dissolution is slower in cold water, so the powder is sifted into a stirred vortex to prevent surface lumps.

The glucan backbone survives standard pasteurization and mildly acidic bases. Extended boiling in strong acid shortens the chain, and enzymes in unpasteurized juice bases can reduce viscosity over time. Protein left on the polymer can brown with reducing sugars, so a sweetened, hot-filled drink darkens faster than a dry blend.

The aroma left in the powder is faint and slightly nutty. Polyphenols from tea or fruit bases can bind to the protein part and add haze, so strongly tannic drinks need a trial at the intended concentration.

Hold-time tests are the practical check. A cold, neutral drink keeps its clarity and body through normal storage, while a warm acidic one slowly loses viscosity. The powder takes up moisture and cakes in open air. No viscosity values are stated for this grade.

Hot water sets the first impression. A cup stirred from this powder looks nearly clear at first and shows a faint haze as the glucan swells over the next minutes, which is a normal sign of hydration and not of instability. Cyclocybe aegerita polysaccharide settles very little on standing, and a gentle stir restores any light haze.

Application Areas

Mushroom Blend Capsules

Cyclocybe aegerita polysaccharide fits capsules next to other mushroom fractions and adds only a faint nutty note. A flow agent prevents bridging in the hopper, and fill weight is checked at the start and end of each run. A desiccant pack protects the finished capsules in humid storage.

Savory Broth Powders

Dry broths can carry the powder at small levels, where it adds a little body after hot-water reconstitution. It is not a flavor source, so seasoning must come from salt, yeast extract or herbs, which do not interfere with the glucan. A small batch is tasted after reconstitution to confirm that the seasoning level still reads right.

Instant Tea-Style Drinks

Dissolved with instant tea granules, the powder adds slight body without much color. Strong tea can bind the protein part and cause haze, so brew strength and polysaccharide level are fixed together in the trial. The cup is judged after it has cooled to drinking temperature.

Stick-Pack Combinations

Pre-blending with sweetener or soluble fiber spreads the powder evenly, so it hydrates without lumps. A barrier laminate prevents caking before use, and a flow check avoids bridging of the fine powder in the sachet hopper.

Granulated Tablet Formats

The powder compresses with standard fillers after dry granulation. Wet granulation turns the surface into a gel and slows drying, so granules are dried to a controlled endpoint and tablet hardness is rechecked after humid storage.

Packaging & Commercial Supply

Essencefield supports product evaluation, formulation trials, commercial orders, and ongoing ingredient supply. Packaging, storage, and shipping arrangements are determined by product characteristics, order quantity, stability requirements, and destination-market needs.

Supply Item	General Information
Sample	Available upon request for formulation, testing, and supplier evaluation
Order Quantities	100 g · 1 kg · 5 kg · 25 kg · 100 kg · 500 kg · Bulk
Sample Lead Time	Typically 5–7 days after sample confirmation
Production Lead Time	Typically 15–30 days after order and specification confirmation
Packaging	Suitable protective inner packaging with bags, cartons, drums, or other appropriate outer packaging
Storage	Store in tightly sealed, suitable protective packaging in a cool, dry environment. Protect from moisture, heat, and direct sunlight. Specific storage conditions follow the approved product specification.
Documentation	COA · Product Specification · TDS · SDS · Applicable declarations (as applicable)

Final order quantities, lead times, packaging, documentation, and shipping arrangements are confirmed according to the selected product and commercial requirements.