Alisol A 24-Acetate 98%

Alisma rhizome (Alisma orientale) is the source of alisol A 24-acetate, a protostane-type triterpenoid in which one side-chain hydroxyl group of alisol A carries an acetyl ester. It is isolated by ethanol extraction and purification and supplied as a white powder that is insoluble in water.

The Primary Grade is 98% alisol A 24-acetate by HPLC, in Food / Dietary Supplement Grade. The figure describes this single acetate, not the free alcohol or a total of alisols, and the identity values in the table apply to the acetate only. Related alisols are listed as separate products.

SKU: BOT-PHY-0056 Category: Phytochemicals Tags: Alisma, Ethanol Extracted, Plant Compounds, Root & Rhizome, Softgels & Oil Fills, Terpenoids

Alisol A 24-Acetate Chemical Identity

Alisol A 24-acetate (C32H52O6, molecular weight 532.8) is the acetyl ester of alisol A (C30H50O5). Both belong to the protostane triterpenoids, a tetracyclic family with a ketone at carbon 3, a hydroxyl at carbon 11 and a branched side chain. In alisol A the side chain carries three hydroxyl groups, and in this compound the one at carbon 24 is acetylated. That ester adds 42 mass units and makes the molecule less polar than the free triol.

The raw material is the rhizome of Alisma orientale (Sam.) Juz. (Alismataceae). Current checklists treat this name as a synonym of Alisma plantago-aquatica, but Alisma orientale remains the trade name. Leaves and the aerial parts are not the source, and other plants called water plantain belong to different genera.

Three neighbors belong in any comparison. Alisol A 98% is the hydrolysis product, Alisma rhizome extract powder holds the full alisol mixture, and Alisma rhizome powder keeps the fiber. Acetate esters of the same alcohol can differ in the position of the acetyl group, so the CAS number and InChIKey in the table fix the compound.

Typical Specifications

Specification Item	Product Information
Natural Source	Alisma orientale rhizome
Plant Part	Root & Rhizome
Processing	Ethanol Extraction & Purification
Compound Class	Terpenoids
Chemical Name (IUPAC)	[(3R,4S,6R)-2,4-dihydroxy-6-[(5R,8S,9S,10S,11S,14R)-11-hydroxy-4,4,8,10,14-pentamethyl-3-oxo-1,2,5,6,7,9,11,12,15,16-decahydrocyclopenta[a]phenanthren-17-yl]-2-methylheptan-3-yl] acetate
CAS Number	18674-16-3
Molecular Formula	C32H52O6
Molecular Weight	532.8 g/mol
PubChem CID	76336194
InChIKey	WXHUQVMHWUQNTG-JSWHPQHOSA-N
Purity	98% (HPLC)
Grade	Food / Dietary Supplement Grade
Appearance	White powder
Solubility / Dispersibility	Insoluble in Water
Quality Parameters
Moisture (LOD)	≤5.0%
Total Ash	≤5.0%
Heavy Metals	Lead ≤2 ppm · Arsenic ≤1 ppm · Cadmium ≤1 ppm · Mercury ≤0.1 ppm
Microbiology	TPC ≤1,000 cfu/g · Yeast & Mold ≤100 cfu/g · E. coli Negative · Salmonella Negative/25 g
Shelf Life	24 months in unopened original packaging, cool and dry storage

Alisol A 24-Acetate Purity & Related Substances

Alisol A 24-acetate 98% is a single-compound grade measured by HPLC. Purity is reported as the area of the acetate peak relative to all detected peaks; water, ash and non-absorbing material fall outside it. Protostane triterpenoids have weak ultraviolet absorption, so methods often use a low detection wavelength or a light-scattering detector, and the choice of detector affects how much of a minor peak is seen.

The closest related substances are the other alisols of the rhizome: alisol A itself, alisol B and its 23-acetate, and the alisol C compounds. Several of them differ by an acetyl group or by one water molecule, which is a small change in mass and polarity, so the method must resolve them by retention time. Positional acetate isomers, where the ester sits on a neighboring hydroxyl, are a second risk, and a standard of the correct isomer is needed.

Acetate esters hydrolyze slowly in damp or alkaline conditions, which returns alisol A. A growing alisol A peak on storage therefore signals moisture. The compound is of plant origin, obtained from the rhizome through ethanol extraction and purification.

For incoming inspection, three checks are practical. A chromatogram with a clean single peak at the expected retention time shows chemical purity, an acetate-specific identity test confirms that the ester is present, and a water determination shows whether the powder has been kept dry. A lot that meets all three is less likely to drift toward alisol A during storage.

Alisol A 24-Acetate Solubility & Delivery

Alisol A 24-acetate is insoluble in water. Its large hydrocarbon skeleton and the acetyl ester leave only two free hydroxyl groups, so it prefers ethanol, methanol and other organic solvents and lipid phases. In aqueous systems the white powder floats or settles and needs a carrier.

Delivery approaches in principle include oil-phase dispersions for softgels, co-solvent premixes, phospholipid or cyclodextrin complexes, surfactant-assisted dispersions and micronized powders. Each approach changes the dissolution rate, not the identity of the compound. The ester group makes neutral pH the safest range, since strong acid or alkali would hydrolyze it.

As a dry powder it is fine, slightly electrostatic and not strongly hygroscopic. Closed containers keep moisture out, and a dilution premix helps accuracy when the dose is small. White powder shows contamination easily, so cleaning between products is checked visually. Opaque containers protect against light, although the compound has no strongly light-sensitive chromophore.

Weighing deserves care because the dose is small. Alisol A 24-acetate is best handled by stepwise dilution into a neutral carrier, checking homogeneity of the premix before it goes into the main blend. This avoids the loss of a fine, light powder on the walls of a large blender.

Application Areas

Hard capsules with premixing

Alisol A 24-acetate is dosed in small weights, so a premix with a free-flowing excipient gives uniform fills. A dry room and closed containers keep the fine powder from clumping, and fill weights are sampled during the run.

Softgels with lipid carriers

The ester dissolves or disperses better in oil than in water, which suits softgels. The fill is stirred during encapsulation and sampled at the start, middle and end of the run to confirm that the insoluble powder has not settled.

Tablets at low inclusion

In tablets the white powder is diluted with filler and binder at low inclusion. A wetting agent helps the matrix disintegrate, and a uniformity check on tablets from the run shows whether the small dose is spread evenly.

Powder sticks with dispersants

Stick packs designed for water rely on a dispersant or hydrocolloid, since the insoluble powder would otherwise float or settle. Sticks are tested for settling and for visible specks after standing in a glass for the time a consumer would take.

Triterpenoid combination blends

Alisol A 24-acetate is combined with other alisols or with Alisma rhizome powder in capsules. Each compound is assayed by its own HPLC method, since percentages from different compounds cannot be added, and the blend is checked for the intended ratio of ester to rhizome matrix.

Packaging & Commercial Supply

Essencefield supports product evaluation, formulation trials, commercial orders, and ongoing ingredient supply. Packaging, storage, and shipping arrangements are determined by product characteristics, order quantity, stability requirements, and destination-market needs.

Supply Item	General Information
Sample	Available upon request for formulation, testing, and supplier evaluation
Order Quantities	100 g · 1 kg · 5 kg · 25 kg · 100 kg · 500 kg · Bulk
Sample Lead Time	Typically 5–7 days after sample confirmation
Production Lead Time	Typically 15–30 days after order and specification confirmation
Packaging	Suitable protective inner packaging with bags, cartons, drums, or other appropriate outer packaging
Storage	Store in tightly sealed, suitable protective packaging in a cool, dry environment. Protect from moisture, heat, and direct sunlight. Specific storage conditions follow the approved product specification.
Documentation	COA · Product Specification · TDS · SDS · Applicable declarations (as applicable)

Final order quantities, lead times, packaging, documentation, and shipping arrangements are confirmed according to the selected product and commercial requirements.