3,5-Dicaffeoylquinic Acid 98%

Known in Chinese literature as isochlorogenic acid A, 3,5-dicaffeoylquinic acid is isolated here from the flower of Lonicera japonica by ethanol extraction and purification. It is quinic acid with caffeic acid esters at positions 3 and 5, and it is a water soluble, off-white powder.

The primary grade is 98% 3,5-dicaffeoylquinic acid by HPLC, in Food / Dietary Supplement Grade. Because five positional isomers share one formula, the locants 3,5 are the identity of this product, and the retention time and spectra of the main peak confirm it.

SKU: BOT-PHY-0047 Category: Phytochemicals Tags: Ethanol Extracted, Flower, Honeysuckle, Plant Compounds, Polyphenols, Water Soluble

3,5-Dicaffeoylquinic Acid Chemical Identity

3,5-Dicaffeoylquinic acid has the molecular formula C25H24O12 and the molecular weight 516.4, and the CAS number is 2450-53-5. In 3,5-dicaffeoylquinic acid the two caffeoyl groups sit at positions 3 and 5 of the quinic acid ring, separated by the free hydroxyl at C4, and the hydroxyl at C1 stays free beside the carboxyl group. The geometry places the two catechol rings on the same face of the ring system but farther apart than in the 3,4 or 4,5 isomers, which affects how the molecule packs and how it elutes.

The old Chinese trade names label 3,5-dicaffeoylquinic acid isochlorogenic acid A, while the 3,4 and 4,5 isomers are isochlorogenic acids B and C. Western papers use the locants, so a buyer who reads both literatures needs the two names side by side. The natural source is the flower of Lonicera japonica Thunb., in which chlorogenic acid is the dominant caffeoylquinic acid and the 3,5 isomer is among the more abundant dicaffeoyl forms. The same flower is also supplied as Honeysuckle Extract 5–25% Chlorogenic Acid, the 10:1 Honeysuckle Flower Extract Powder and the milled Honeysuckle Flower Powder. Those materials are defined by other markers, while this acid is one isomer. Quality teams that buy several of them keep the labels apart, because a certificate for the mixed extract does not describe single 3,5-dicaffeoylquinic acid, and a certificate for the single compound says little about the extract.

Typical Specifications

Specification Item	Product Information
Natural Source	Lonicera japonica flower
Plant Part	Flower
Processing	Ethanol Extraction & Purification
Compound Class	Polyphenols
CAS Number	2450-53-5
Molecular Formula	C25H24O12
Molecular Weight	516.4
Purity	98% (HPLC)
Grade	Food / Dietary Supplement Grade
Appearance	Off-White powder
Solubility / Dispersibility	Water Soluble
Quality Parameters
Moisture (LOD)	≤5.0%
Total Ash	≤5.0%
Heavy Metals	Lead ≤2 ppm · Arsenic ≤1 ppm · Cadmium ≤1 ppm · Mercury ≤0.1 ppm
Microbiology	TPC ≤1,000 cfu/g · Yeast & Mold ≤100 cfu/g · E. coli Negative · Salmonella Negative/25 g
Shelf Life	24 months in unopened original packaging, cool and dry storage

3,5-Dicaffeoylquinic Acid Purity Grades & Impurities

The 98% figure is an HPLC result near 325 nm that compares the 3,5-dicaffeoylquinic acid peak with the other detected peaks. Water, ash and compounds without a response are outside the figure, which is why the table limits moisture and ash separately. One grade is offered for this isomer.

Isomer resolution is the first analytical requirement. On a reversed-phase column the 3,5 isomer usually elutes between the 3,4 and 4,5 forms, so it has a close neighbor on each side, and a gradient that is too steep merges them. A trace that separates all three, with the 1,3 and 1,5 isomers elsewhere in the run, confirms that the main peak is the 3,5 compound. The report should also show chlorogenic acid and its isomers as separate, earlier peaks.

The 3,5 isomer has a special relationship with the others. Acyl migration, the shift of a caffeoyl group to a neighboring hydroxyl, can move one ester of the 3,5 isomer onto the C4 hydroxyl to give the 3,4 or 4,5 form. The process is driven by heat and by neutral or alkaline pH, so a lot that was held warm in solution will show the neighbors growing at the expense of the main peak. Samples are prepared fresh in a mildly acidic solvent for that reason.

Hydrolysis is the second route of change, and it releases caffeic acid and monocaffeoylquinic acids. A clean powder is off-white, and a tan tone suggests catechol oxidation or flower pigment. Moisture and ash are limited separately, and storage is dry, sealed and dark.

3,5-Dicaffeoylquinic Acid Solubility & Delivery

Water, aqueous ethanol and methanol dissolve 3,5-dicaffeoylquinic acid, and it is poorly soluble in nonpolar solvents. Its carboxyl group and free hydroxyls make it polar, and the solution is slightly acidic and pale yellow. Solubility rises with warmth and with pH, although higher pH also speeds hydrolysis and oxidation.

Delivery of 3,5-dicaffeoylquinic acid therefore concentrates on stability. Drink bases are kept mildly acidic, thermal steps are short, and a chelating agent counters traces of iron and copper that would darken the liquid. The powder is hygroscopic, so a closed container and a desiccant protect flow and assay between uses. Because the compound absorbs light and isomerizes at the double bonds, amber glass is used for solutions. Premixes with a dry carrier give accurate small weights, and anti-static tools help with the fine, clinging powder.

Application Areas

Mildly Acidic Drink Concentrates

3,5-Dicaffeoylquinic acid dissolves readily in water, and a pH around the acidic end of the drink range keeps it stable. Cool-fill the concentrate, avoid long holds above room temperature, and use deionized water to keep iron out of the formula.

Capsule Fills in Dry Rooms

Capsules take 3,5-dicaffeoylquinic acid at low inclusion. Because the powder takes up moisture, the filling room is kept dry, a flow agent is blended in, and the finished bottle carries a desiccant to protect the fill weight over the shelf life.

Mixed-Isomer Stick Premixes

Sticks carry 3,5-dicaffeoylquinic acid in a dry premix with a sweetener and a fruit acid. The premix is sieved to avoid lumps, and flavor choice is guided by the bitter, astringent edge of the ester, which fruit and mint notes cover well.

Honeysuckle Component Blends

Blends with honeysuckle extracts pair the single isomer with the mixed material. Each ingredient is weighed on its own, and the HPLC trace of the blend, run on a column that resolves all five isomers, confirms that the pattern is as intended.

Isomer Reference Work

Analytical teams use the 98% 3,5-dicaffeoylquinic acid grade to anchor the middle of the three-isomer cluster in method development. The standard is stored dry and cold, and a retained sample from each lot preserves the retention-time record.

Packaging & Commercial Supply

Essencefield supports product evaluation, formulation trials, commercial orders, and ongoing ingredient supply. Packaging, storage, and shipping arrangements are determined by product characteristics, order quantity, stability requirements, and destination-market needs.

Supply Item	General Information
Sample	Available upon request for formulation, testing, and supplier evaluation
Order Quantities	100 g · 1 kg · 5 kg · 25 kg · 100 kg · 500 kg · Bulk
Sample Lead Time	Typically 5–7 days after sample confirmation
Production Lead Time	Typically 15–30 days after order and specification confirmation
Packaging	Suitable protective inner packaging with bags, cartons, drums, or other appropriate outer packaging
Storage	Store in tightly sealed, suitable protective packaging in a cool, dry environment. Protect from moisture, heat, and direct sunlight. Specific storage conditions follow the approved product specification.
Documentation	COA · Product Specification · TDS · SDS · Applicable declarations (as applicable)

Final order quantities, lead times, packaging, documentation, and shipping arrangements are confirmed according to the selected product and commercial requirements.