Acacetin 98%

A yellow flavone from the flower of Chrysanthemum indicum, acacetin is the 4′-methyl ether of apigenin, isolated by ethanol extraction and purification. It is supplied as an insoluble yellow powder at a single 98% grade.

The Primary Grade is 98% acacetin by HPLC, in Food / Dietary Supplement Grade. Acacetin is the sugar-free core of the flavone glycoside linarin, so the product is an aglycone and not a glycoside. The identity data match the free compound, and the powder is insoluble in water.

SKU: BOT-PHY-0052 Category: Phytochemicals Tags: Ethanol Extracted, Flavonoids, Flower, Plant Compounds, Softgels & Oil Fills, Wild Chrysanthemum

Acacetin Chemical Identity

Acacetin (5,7-dihydroxy-4′-methoxyflavone, C16H12O5, molecular weight 284.26) is a flavone: the same three-ring skeleton as apigenin, but with a methoxy group in place of the hydroxyl on the B ring. That single methyl ether lowers polarity and changes metabolism and stability compared with apigenin. In wild chrysanthemum flowers acacetin is found mainly bound to sugar as linarin, the 7-O-rutinoside, and the free aglycone is obtained after the sugar is removed. The natural source is the flower of Chrysanthemum indicum L. (Asteraceae), the wild chrysanthemum, which is a different species from the cultivated C. morifolium. The same formula C16H12O5 is shared with biochanin A, an isoflavone, so mass alone cannot identify the compound. Buyers comparing neighboring products can look at wild chrysanthemum flower powder and Linarin 98%. The glycoside and the aglycone are different compounds with their own identifiers and solubility.

Specifications often mix up the compound with its glycoside. Linarin is acacetin carrying a rutinose sugar, so it is heavier, more polar and used as the marker for wild chrysanthemum in pharmacopoeial work. The acacetin 98% grade is the free flavone, and a purchase order should quote the CAS number 480-44-4 and the single-peak HPLC purity so that the two are not exchanged.

Typical Specifications

Specification Item	Product Information
Natural Source	Chrysanthemum indicum flower
Plant Part	Flower
Processing	Ethanol Extraction & Purification
Compound Class	Flavonoids, Polyphenols
Chemical Name (IUPAC)	5,7-dihydroxy-2-(4-methoxyphenyl)chromen-4-one
CAS Number	480-44-4
Molecular Formula	C16H12O5
Molecular Weight	284.26 g/mol
PubChem CID	5280442
InChIKey	DANYIYRPLHHOCZ-UHFFFAOYSA-N
Purity	98% (HPLC)
Grade	Food / Dietary Supplement Grade
Appearance	Yellow powder
Solubility / Dispersibility	Insoluble in Water
Quality Parameters
Moisture (LOD)	≤5.0%
Total Ash	≤5.0%
Heavy Metals	Lead ≤2 ppm · Arsenic ≤1 ppm · Cadmium ≤1 ppm · Mercury ≤0.1 ppm
Microbiology	TPC ≤1,000 cfu/g · Yeast & Mold ≤100 cfu/g · E. coli Negative · Salmonella Negative/25 g
Shelf Life	24 months in unopened original packaging, cool and dry storage

Acacetin Purity & Related Substances

Acacetin 98% is a single grade measured by HPLC, and it shows the acacetin peak as a share of all detected peaks. Water, ash and non-absorbing material are not counted. The related substances in the flower are apigenin, luteolin and their glycosides, with linarin as the principal acacetin glycoside. Apigenin differs by one methyl group and elutes earlier on a reversed-phase column, so a method that resolves the pair avoids over-stating purity. Residual linarin indicates incomplete hydrolysis and appears as a separate, more polar peak. Biochanin A shares the formula and mass, and its separation relies on retention time and on the InChIKey, not on mass alone. Ethanol extraction of the flower and a purification step define the route, and no other route is described. Total flavonoids by colorimetry is a different measurement and cannot be used in place of the HPLC figure for acacetin. A yellow-green cast or a dark powder points to oxidation or residual plant pigments and justifies retesting.

For incoming checks, a diode-array detector helps because flavones share a similar UV profile. Acacetin and apigenin have maxima at close wavelengths, so retention time and a reference standard carry the identification. A chromatogram that shows only one peak at the expected retention time, with a clean baseline, is the practical release criterion.

Reference standards matter as much as the column. A standard of acacetin from a recognized source, with the same CAS number, is the correct comparison, and a standard labelled only as a flavone mixture would give a misleading assay. Different detection wavelengths shift the area percentages slightly, so the same wavelength should be used when two lots are compared.

Acacetin Solubility & Delivery

Acacetin is insoluble in water. The methyl ether removes one hydrogen-bond donor compared with apigenin, so it is even less polar and dissolves best in warm ethanol and polar aprotic solvents, with little solubility in cold aqueous media. The flat structure packs well in the crystal, which slows dissolution. Practical delivery in principle uses micronized powders, lipid carriers, phospholipid or cyclodextrin complexes, and surfactant-assisted dispersions. Each raises the apparent dissolution rate without changing the molecule. The compound is stable when dry and kept closed, and it degrades slowly under strong light or alkaline pH. The fine yellow powder is electrostatic and forms dust, so closed transfer helps. Because inclusion is low, premixing and weighing accuracy are more influential than the choice of capsule shell. Wetting the powder in a small volume of carrier before the main mix avoids floating lumps in liquid systems.

In dry blends acacetin behaves like other flavone aglycones: it is stable, slightly electrostatic and non-hygroscopic when dry. Static control and closed transfer reduce dust and weighing loss. In liquids it floats unless wetted, so a prewetting step in a small volume of carrier is recommended before the main mix.

Cleaning is simple because the powder does not stain heavily, but acacetin residues are hard to wash from stainless steel with water alone, so a short ethanol rinse between products avoids carry-over. Dust extraction at the weighing station also reduces losses of the fine, electrostatic powder.

Application Areas

Hard capsules with a premix

Acacetin is dosed in small amounts, so a premix with a free-flowing excipient gives reliable fill weights. A dry room and closed containers keep the powder from clumping before the shell is closed, and fills are sampled during the run.

Softgels with lipid carriers

A micronized acacetin dispersion in oil or a phospholipid-containing carrier wets better than a dry fill. The fill is sampled for uniformity before encapsulation because the insoluble powder tends to settle in an unstirred tank.

Tablets at low inclusion

In tablets the yellow powder is diluted with filler and binder and compressed at low inclusion. A uniformity check confirms the small dose is distributed evenly across the batch, and coating hides any speckling on the surface.

Powder sticks and flavone blends

Dry mixes stirred into water need a dispersant so that the insoluble powder remains suspended. In blends with apigenin or linarin, each component is assayed separately because HPLC percentages from different compounds cannot be added.

Packaging & Commercial Supply

Essencefield supports product evaluation, formulation trials, commercial orders, and ongoing ingredient supply. Packaging, storage, and shipping arrangements are determined by product characteristics, order quantity, stability requirements, and destination-market needs.

Supply Item	General Information
Sample	Available upon request for formulation, testing, and supplier evaluation
Order Quantities	100 g · 1 kg · 5 kg · 25 kg · 100 kg · 500 kg · Bulk
Sample Lead Time	Typically 5–7 days after sample confirmation
Production Lead Time	Typically 15–30 days after order and specification confirmation
Packaging	Suitable protective inner packaging with bags, cartons, drums, or other appropriate outer packaging
Storage	Store in tightly sealed, suitable protective packaging in a cool, dry environment. Protect from moisture, heat, and direct sunlight. Specific storage conditions follow the approved product specification.
Documentation	COA · Product Specification · TDS · SDS · Applicable declarations (as applicable)

Final order quantities, lead times, packaging, documentation, and shipping arrangements are confirmed according to the selected product and commercial requirements.